Article

Construction of a randomly barcoded insertional mutant library in the filamentous fungus <i>Trichoderma atroviride</i>

2025-12-01

Abstract excerpt

Filamentous fungi play key roles in ecosystems, agriculture, biotechnology, symbiosis, and disease, yet the large-scale characterization of gene function in these organisms remains limited by low transformation efficiencies and their multinucleate, syncytial cells, which complicate high-throughput screening strategies. To address the challenge of high-throughput screening in filamentous fungi, we developed methods...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0c62e9ca-f00d-5469-bfe8-06a19f0d0124
DOI: 10.1101/2025.11.30.691285

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Construction of a randomly barcoded insertional mutant library in the filamentous fungus <i>Trichoderma atroviride</i>DOI 10.1101/2025.11.30.691285Read

Complete Genome Sequences and Genome-Wide Characterization of Trichoderma Biocontrol Agents Provide New Insights into their Evolution and Variation in Genome Organization, Sexual Development, and Fungal-Plant Interactions.Semantic proximity Read

Whole-Genome Sequencing and Comparative Genomic Analysis of Potential Biotechnological Strains of Trichoderma harzianum, Trichoderma atroviride <i>, and</i> Trichoderma reeseiSemantic proximity Read

Development of genome-editing technologies for plant pathogenic fungiSemantic proximity Read

Random insertional mutagenesis in fungal genomes to identify virulence factors.Semantic proximity Read

An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus.Semantic proximity Read

Development of a high throughput transformation system for insertional mutagenesis in Magnaporthe oryzae.Semantic proximity Read

Advancing functional genetics through <i>Agrobacterium</i> -mediated insertional mutagenesis and CRISPR/Cas9 in the commensal and pathogenic yeast <i>Malassezia furfur</i>Semantic proximity Read

Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant.Semantic proximity Read

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein.Semantic proximity Read

Identifying genes in Fusarium verticillioides through forward and reverse genetics.Semantic proximity Read

Rapid mapping of insertional mutations to probe cell wall regulation in Cryptococcus neoformans.Semantic proximity Read

Large-scale insertional mutagenesis in Magnaporthe oryzae by Agrobacterium tumefaciens-mediated transformation.Semantic proximity Read