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MIDAS: A Modular DNA Assembly System for Synthetic Biology.

ACS synthetic biology - 20 Apr 2018

van Dolleweerd Craig J, Kessans Sarah A, Van de Bittner Kyle C, Bustamante Leyla Y, Bundela Rudranuj, Scott Barry, Nicholson Matthew J, Parker Emily J

Abstract excerpt

A modular and hierarchical DNA assembly platform for synthetic biology based on Golden Gate (Type IIS restriction enzyme) cloning is described. This enabling technology, termed MIDAS (for Modular Idempotent DNA Assembly System), can be used to precisely assemble multiple DNA fragments in a single reaction using a standardized assembly design. It can be used to build genes from libraries of sequence-verified,...

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PMID: 29620866
DOI: 10.1021/acssynbio.7b00363

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