Article
Generation of interleukin-2 receptor gamma gene knockout pigs from somatic cells genetically modified by zinc finger nuclease-encoding mRNA.
PloS one - 1 Jan 2013
Watanabe Masahito, Nakano Kazuaki, Matsunari Hitomi, Matsuda Taisuke, Maehara Miki, Kanai Takahiro, Kobayashi Mirina, Matsumura Yukina, Sakai Rieko, Kuramoto Momoko, Hayashida Gota, Asano Yoshinori, Takayanagi Shuko, Arai Yoshikazu, Umeyama Kazuhiro, Nagaya Masaki, Hanazono Yutaka, Nagashima Hiroshi
Abstract excerpt
Zinc finger nuclease (ZFN) is a powerful tool for genome editing. ZFN-encoding plasmid DNA expression systems have been recently employed for the generation of gene knockout (KO) pigs, although one major limitation of this technology is the use of potentially harmful genome-integrating plasmid DNAs. Here we describe a simple, non-integrating strategy for generating KO pigs using ZFN-encoding mRNA. The...
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