Article
Construction and characterization in vivo of Bordetella pertussis aroA mutants.
Infection and immunity - 1 Mar 1990
Roberts M, Maskell D, Novotny P, Dougan G
Abstract excerpt
A DNA fragment encoding a kanamycin resistance determinant was used to insertionally inactivate the cloned aroA gene of Bordetella pertussis in Escherichia coli K-12, and a conjugative shuttle vector system based on the suicide vector pRTP1 was used to deliver the mutations from E. coli back into...
Topics
- Aerosols
- Animals
- Antibodies, Bacterial
- Bacterial Vaccines
- Bordetella pertussis
- Escherichia coli
- Female
- Genotype
- Lung
- Mice
- Mutation
- Phenotype
- Vaccination
- Vaccines, Attenuated