Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of Bordetella pertussis strains that overproduce genetically inactivated pertussis toxin.

Applied and environmental microbiology - 1 Jan 1992

Zealey G R, Loosmore S M, Yacoob R K, Cockle S A, Herbert A B, Miller L D, Mackay N J, Klein M H

Abstract excerpt

Nontoxic analogs of pertussis toxin (PT), produced by in vitro mutagenesis of the tox operon, are immunogenic and protective against infection by Bordetella pertussis. The moderate levels of PT production by B. pertussis, however, make it the limiting antigen in the formulation of multicomponent, acellular, recombinant whooping cough vaccines. To increase production of the highly detoxified Lys9Gly129 PT analog...

Topics

Alleles
Blotting, Southern
Bordetella pertussis
Chromatography, High Pressure Liquid
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Kinetics
Operon
Pertussis Toxin
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Virulence Factors, Bordetella

Source

Open in PubMed Open full text

Identifiers

PMID: 1539974
DOI: 10.1128/aem.58.1.208-214.1992
PMCID: PMC195193

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1539974-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of Bordetella pertussis strains that overproduce genetically inactivated pertussis toxin.PMID 1539974Read ↗

Engineering of genetically detoxified pertussis toxin analogs for development of a recombinant whooping cough vaccine.Semantic proximity Read ↗

Gene replacement in Bordetella pertussis by transformation with linear DNA.Semantic proximity Read ↗

Development of non-toxigenic vaccine strains of Bordetella pertussis by gene replacement.Semantic proximity Read ↗

A versatile, non genetically modified organism (GMO)-based strategy for controlling low-producer mutants in Bordetella pertussis cultures using antigenic modulation.Semantic proximity Read ↗

Four single-basepair mutations in the ptx promoter of Bordetella bronchiseptica are sufficient to activate the expression of pertussis toxin.Semantic proximity Read ↗

Genetically derived toxoids for use as vaccines and adjuvants.Semantic proximity Read ↗

Recombinant acellular pertussis vaccine--from the laboratory to the clinic: improving the quality of the immune response.Semantic proximity Read ↗

Mutants in the ptlA-H genes of Bordetella pertussis are deficient for pertussis toxin secretion.Semantic proximity Read ↗

Genetic approaches to a vaccine for pertussis.Semantic proximity Read ↗

Transfer of a pertussis toxin expression locus to isogenic bvg-positive and bvg-negative strains of Bordetella bronchiseptica using an in vivo technique.Semantic proximity Read ↗

Multivalent Chromosomal Expression of the Clostridium botulinum Serotype A Neurotoxin Heavy-Chain Antigen and the Bacillus anthracis Protective Antigen in Lactobacillus acidophilusSemantic proximity Read ↗

Investigating pertussis toxin and its impact on vaccination.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.