Article
Construction and vaccine potential of an aroA mutant of Pasteurella haemolytica.
Veterinary microbiology - 1 Sept 1994
Homchampa P, Strugnell R A, Adler B
Abstract excerpt
The aroA gene, encoding 5-enolpyruvylshikimate 3-phosphate synthase, from Pasteurella haemolytica biotype A, serotype 1 was cloned by complementation of the aroA mutation in Escherichia coli strain AB2829 after electroporation with a DNA library constructed in pUC18. The cloned P. haemolytica aroA gene was inactivated by insertion of a kanamycin resistance gene and reintroduced by allelic exchange into the...
Topics
- Animals
- Bacteremia
- Bacterial Typing Techniques
- Bacterial Vaccines
- Blotting, Southern
- Cloning, Molecular
- Disease Models, Animal
- Electroporation
- Escherichia coli
- Female
- Immunoenzyme Techniques