Article
Construction and preliminary immunobiological characterization of a novel, non-reverting, intranasal live attenuated whooping cough vaccine candidate.
Journal of microbiology and biotechnology - 1 Jun 2012
Cornford-Nairns Renee, Daggard Grant, Mukkur Trilochan
Abstract excerpt
We describe the construction and immunobiological properties of a novel whooping cough vaccine candidate, in which the aroQ gene, encoding 3-dehydroquinase, was deleted by insertional inactivation using the kanamycin resistance gene cassette and allelic exchange using a Bordetella suicide vector. The aroQ B. pertussis mutant required supplementation of media to grow but failed to grow on an unsupplemented medium....
Topics
- Administration, Intranasal
- Animals
- Antibodies, Bacterial
- Bordetella pertussis
- Culture Media
- Cytokines
- Disease Models, Animal
- Hydro-Lyases
- Immunoglobulin G
- Leukocytes, Mononuclear
- Lung
- Mice
- Mutation
- Pertussis Vaccine
- Trachea
- Vaccination
- Vaccines, Attenuated
- Virulence Factors