Article
An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in mice.
Microbiology (Reading, England) - 1 Jul 1996
Oyston P C, Russell P, Williamson E D, Titball R W
Abstract excerpt
This study describes a PCR-based approach for the production of a rationally attenuated mutant of Yersinia pestis. Degenerate primers were used to amplify a fragment encoding 91.45% of the aroA gene of Y. pestis MP6 which was cloned into pUC18. The remainder of the gene was isolated by inverse PCR. The gene was sequenced and a restriction map was generated. The Y. pestis aroA gene had 75.9% identity with the aroA...
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