Article
Site-directed mutagenesis of the -10 region of the lacUV5 promoter. Introduction of dA4.dT4 tract suppresses open complex formation.
The Journal of biological chemistry - 5 Mar 1990
Chan P T, Lebowitz J
Abstract excerpt
Homopolymeric dAn.dTn sequences, where n is 4 or greater, have special properties leading to increased duplex stability and DNA bending. The lacUV5 promoter was used to examine the functional consequences of changing the -10 TATAAT consensus sequence to the sequence TAAAAT. The transversion mutation at the underlined site was accomplished with site-directed mutagenesis using translation termination as the...
Topics
- Base Sequence
- Coliphages
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Deoxyribonuclease I
- Escherichia coli
- Genetic Vectors
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleotide Mapping