Article
Mapping of T7 RNA polymerase active site with novel reagents--oligonucleotides with reactive dialdehyde groups.
FEBS letters - 8 Jan 1999
Tunitskaya V L, Rusakova E E, Memelova L V, Kochetkov S N, Van Aerschot A, Herdewijn P, Efimtseva E V, Ermolinsky B S, Mikhailov S N
Abstract excerpt
Oligonucleotides of a novel type containing 2'-O-beta-ribofuranosyl-cytidine were synthesized and further oxidized to yield T7 consensus promoters with dialdehyde groups. Both types of oligonucleotides were tested as templates, inhibitors, and affinity reagents for T7 RNA polymerase and its mutan...
Topics
- Affinity Labels
- Aldehydes
- Bacteriophage T7
- Base Sequence
- Catalytic Domain
- DNA-Directed RNA Polymerases
- Indicators and Reagents
- Mutation
- Oligodeoxyribonucleotides
- Promoter Regions, Genetic
- Viral Proteins