Article
Mapping of T7 RNA polymerase active site with novel reagents--oligonucleotides with reactive dialdehyde groups.
FEBS letters - 8 Jan 1999
Tunitskaya V L, Rusakova E E, Memelova L V, Kochetkov S N, Van Aerschot A, Herdewijn P, Efimtseva E V, Ermolinsky B S, Mikhailov S N
Abstract excerpt
Oligonucleotides of a novel type containing 2'-O-beta-ribofuranosyl-cytidine were synthesized and further oxidized to yield T7 consensus promoters with dialdehyde groups. Both types of oligonucleotides were tested as templates, inhibitors, and affinity reagents for T7 RNA polymerase and its mutants. All oligonucleotides tested retained high affinity towards the enzyme. Wild-type T7 RNA polymerase and most of the...
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