Effect of mutations in the "extended -10" motif of three Bacillus subtilis sigmaA-RNA polymerase-dependent promoters.

doi:10.1006/jmbi.1998.2526

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Article

Effect of mutations in the "extended -10" motif of three Bacillus subtilis sigmaA-RNA polymerase-dependent promoters.

Journal of molecular biology - 26 Feb 1999

Camacho A, Salas M

Abstract excerpt

The "extended -10" motif described originally in Escherichia coli promoters occurs frequently in other bacterial promoters. Most Bacillus subtilis bacteriophage o29 promoters contain this motif. To analyse the influence of the motif on sigmaA-RNA polymerase transcription, the 5'-TG-3' dinucleotide was changed to 5'-AC-3' in three o29 promoters. This change impaired RNA polymerase binding to the promoters; the...

Topics

Bacillus subtilis
Bacterial Proteins
Consensus Sequence
DNA Footprinting
DNA, Bacterial
DNA-Binding Proteins
DNA-Directed RNA Polymerases
Mutation
Promoter Regions, Genetic
Sigma Factor
Transcription, Genetic

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Identifiers

PMID: 10024443
DOI: 10.1006/jmbi.1998.2526

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