Article
Kinetic studies of the modulation of ada promoter activity by upstream elements.
The EMBO journal - 1 Jul 1990
Bertrand-Burggraf E, Dunand J, Fuchs R P, Lefèvre J F
Abstract excerpt
We have determined the kinetics of initiation of transcription of the wild-type ada promoter by abortive initiation assays. In the absence of activation, it is a weak promoter, with an association constant KB and an isomerization rate constant k2 comparable to those obtained under the same condit...
Topics
- Base Sequence
- DNA Topoisomerases, Type I
- DNA-Directed RNA Polymerases
- Escherichia coli
- Kinetics
- Methyltransferases
- Molecular Sequence Data
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase
- Oligonucleotide Probes
- Plasmids
- Promoter Regions, Genetic
- Protein Binding
- Transcription, Genetic