Article
Kinetic studies of the modulation of ada promoter activity by upstream elements.
The EMBO journal - 1 Jul 1990
Bertrand-Burggraf E, Dunand J, Fuchs R P, Lefèvre J F
Abstract excerpt
We have determined the kinetics of initiation of transcription of the wild-type ada promoter by abortive initiation assays. In the absence of activation, it is a weak promoter, with an association constant KB and an isomerization rate constant k2 comparable to those obtained under the same conditions for other positively regulated promoters (0.36 x 10(7) M-1 and 1.7 x 10(-2) s-1, respectively, at 37 degrees C and...
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