Article
Biophysical studies of engineered mutant proteins based on calbindin D9k modified in the pseudo EF-hand.
European journal of biochemistry - 26 Jan 1990
Johansson C, Brodin P, Grundström T, Thulin E, Forsén S, Drakenberg T
Abstract excerpt
The genes for four mutant proteins from calbindin D9k, all with mutations in the N-terminal Ca2+-binding domain (pseudo EF-hand) have been synthesized and expressed in Escherichia coli. The purification scheme has been modified to minimize the formation of deamidated proteins. The set of modifica...
Topics
- Alanine
- Animals
- Binding Sites
- Calbindins
- Calcium
- Escherichia coli
- Gene Expression
- Glycine
- Kinetics
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Mutation
- Proline
- Protein Conformation
- Protein Engineering
- Recombinant Proteins
- S100 Calcium Binding Protein G
- Temperature