Article
Stimulation of intestinal basolateral membrane calcium-pump activity by recombinant synthetic calbindin-D9k and specific mutants.
Biochemical and biophysical research communications - 31 Jul 1990
Walters J R, Howard A, Charpin M V, Gniecko K C, Brodin P, Thulin E, Forsén S
Abstract excerpt
Calcium transport by the Ca2(+)-pumping ATPase in rat duodenal basolateral-enriched membrane vesicles was stimulated by synthetic calbindin-D9k in a similar fashion to the purified natural protein. In order to elucidate the mechanism of this effect, various synthetic mutant proteins were studied. Proteins with modifications to the N-terminal Ca2(+)-binding domain, or to a cluster of negatively-charged surface...
Topics
- Animals
- Biological Transport, Active
- Calbindins
- Calcium
- Calcium Channels
- Cell Membrane
- Duodenum
- Male
- Mutation
- Rats
- Recombinant Proteins