Article
Human recombinant alpha-parvalbumin and nine mutants with individually inactivated calcium- and magnesium-binding sites: biochemical and immunological properties.
Biochimica et biophysica acta - 11 Oct 1996
Rhyner J A, Durussel I, Cox J A, Ilg E C, Schäfer B W, Heizmann C W
Abstract excerpt
Human recombinant alpha-parvalbumin (PVwt) and nine mutant proteins, containing inactivating substitutions at positions essential for Ca2+ binding in the CD Ca(2+)-binding site (PVE62V, PVD51A, PVD51A,62V), the EF site (PVE101V, PVD90A, PVD90A,E101V) or in both (PVE62V,E101V, PVD51A,D90A, PVD51A,...
Topics
- Animals
- Antibody Specificity
- Binding Sites
- Calcium
- Cell Extracts
- Cerebellum
- Escherichia coli
- Humans
- Immune Sera
- Isoelectric Point
- Magnesium
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis, Site-Directed
- Mutation
- Parvalbumins
- Protein Binding
- Rabbits