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Article

Design and synthesis of the pseudo-EF hand in calbindin D9K: effect of amino acid substitutions in the alpha-helical regions.

Proteins - 1 Jan 1991

Tsuji T, Kaiser E T

Abstract excerpt

A series of 37-residue analogues of the pseudo-EF hand in bovine calbindin D9K has been synthesized by the solid phase method. In the presence of calcium an alpha-helical induction of up to 44% was observed for the peptide with the native sequence with a Kd for calcium binding of 0.35 mM. A number of amino acid substitutions have been carried out to study the packing of the two alpha-helices based on the crystal...

Topics

Amino Acid Sequence
Calbindins
Calcium Chloride
Circular Dichroism
Disulfides
Drug Design
Molecular Sequence Data
Mutation
Protein Conformation
S100 Calcium Binding Protein G
Solubility

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Spectrometry, Fluorescence

Source

Identifiers

PMID: 2017432
DOI: 10.1002/prot.340090103

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2017432-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Design and synthesis of the pseudo-EF hand in calbindin D9K: effect of amino acid substitutions in the alpha-helical regions.PMID 2017432Read ↗

Biophysical studies of engineered mutant proteins based on calbindin D9k modified in the pseudo EF-hand.Semantic proximity Read ↗

Designing sequence to control protein function in an EF-hand protein.Semantic proximity Read ↗

The EF-hand domain: a globally cooperative structural unit.Semantic proximity Read ↗

Functional properties of calbindin D9K mutants with exchanged Ca2+ binding sites.Semantic proximity Read ↗

Characterization of the N-terminal half-saturated state of calbindin D9k: NMR studies of the N56A mutant.Semantic proximity Read ↗

Sequence and context dependence of EF-hand loop dynamics. An 15N relaxation study of a calcium-binding site mutant of calbindin D9k.Semantic proximity Read ↗

Quantitative measurements of the cooperativity in an EF-hand protein with sequential calcium binding.Semantic proximity Read ↗

An extended hydrophobic core induces EF-hand swapping.Semantic proximity Read ↗

Disulfide bonds in homo- and heterodimers of EF-hand subdomains of calbindin D9k: stability, calcium binding, and NMR studies.Semantic proximity Read ↗

Computer-aided design of beta-sheet peptides.Semantic proximity Read ↗

Solvation energetics and conformational change in EF-hand proteins.Semantic proximity Read ↗

Remodeling of the AB site of rat parvalbumin and oncomodulin into a canonical EF-hand.Semantic proximity Read ↗

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