Article
Directed evolution of Mycobacterium tuberculosis β-lactamase reveals gatekeeper residue that regulates antibiotic resistance and catalytic efficiency.
PloS one - 1 Jan 2013
Feiler Christian, Fisher Adam C, Boock Jason T, Marrichi Matthew J, Wright Lori, Schmidpeter Philipp A M, Blankenfeldt Wulf, Pavelka Martin, DeLisa Matthew P
Abstract excerpt
Directed evolution can be a powerful tool for revealing the mutational pathways that lead to more resistant bacterial strains. In this study, we focused on the bacterium Mycobacterium tuberculosis, which is resistant to members of the β-lactam class of antibiotics and thus continues to pose a major public health threat. Resistance of this organism is the result of a chromosomally encoded, extended spectrum class...
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