Article
Mutational analysis of a red fluorescent protein-based calcium ion indicator.
Sensors (Basel, Switzerland) - 2 Sept 2013
Carlson Haley J, Campbell Robert E
Abstract excerpt
As part of an ongoing effort to develop genetically encoded calcium ion (Ca2+) indicators we recently described a new variant, designated CH-GECO2.1, that is a genetic chimera of the red fluorescent protein (FP) mCherry, calmodulin (CaM), and a peptide that binds to Ca2+-bound CaM. In contrast to the closely related Ca2+ indicator R-GECO1, CH-GECO2.1 is characterized by a much higher affinity for Ca2+ and a...
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