Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry.

Protein engineering, design & selection : PEDS - 1 Dec 2013

Carlson Haley J, Campbell Robert E

Abstract excerpt

Red fluorescent indicators for calcium ion (Ca(2+)) are preferable, relative to blue-shifted alternatives, for biological imaging applications due to the lower phototoxicity, lower autofluorescent background and deeper tissue penetration associated with longer wavelength light. Accordingly, we undertook the development of a genetically encoded Ca(2+) indicator based on the popular and widely utilized...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Calcium Channels
Share to Calmodulin
Share to Cytological Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Directed Molecular Evolution

Share to Luminescent Proteins

Share to Microscopy, Fluorescence

Share to Protein Engineering

Share to Red Fluorescent Protein

Source

Identifiers

PMID: 24151339
DOI: 10.1093/protein/gzt052

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry.PMID 24151339Read ↗

Identification of sites within a monomeric red fluorescent protein that tolerate peptide insertion and testing of corresponding circular permutations.Semantic proximity Read ↗

Circularly permuted green fluorescent proteins engineered to sense Ca2+.Semantic proximity Read ↗

Creation of circularly permutated yellow fluorescent proteins using fluorescence screening and a tandem fusion template.Semantic proximity Read ↗

Generation of longer emission wavelength red fluorescent proteins using computationally designed libraries.Semantic proximity Read ↗

Significant expansion of the fluorescent protein chromophore through the genetic incorporation of a metal-chelating unnatural amino acid.Semantic proximity Read ↗

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calciumSemantic proximity Read ↗

Mutational analysis of a red fluorescent protein-based calcium ion indicator.Semantic proximity Read ↗

High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant.Semantic proximity Read ↗

Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution.Semantic proximity Read ↗

Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria.Semantic proximity Read ↗

Enhancing fluorescent protein photostability through robot-assisted photobleaching.Semantic proximity Read ↗

Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.