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Article

High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant.

Communications biology - 8 Jun 2024

Hara Yusuke, Ichiraku Aya, Matsuda Tomoki, Sakane Ayuko, Sasaki Takuya, Nagai Takeharu, Horikawa Kazuki

Abstract excerpt

Genetically encoded Ca2+ indicators (GECIs) are versatile for live imaging of cellular activities. Besides the brightness and dynamic range of signal change of GECIs, Ca2+ affinity is another critical parameter for successful Ca2+ imaging, as the concentration range of Ca2+ dynamics differs from low nanomolar to sub-millimolar depending on the celltype and organism. However, ultrahigh-affinity GECIs, particularly...

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PMID: 38851844
DOI: 10.1038/s42003-024-06394-0
PMCID: PMC11162441

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