Article
Platelet endothelial cell adhesion molecule targeted oxidant-resistant mutant thrombomodulin fusion protein with enhanced potency in vitro and in vivo.
The Journal of pharmacology and experimental therapeutics - 1 Nov 2013
Carnemolla Ronald, Greineder Colin F, Chacko Ann-Marie, Patel Kruti Rajan, Ding Bi-Sen, Zaitsev Sergei, Esmon Charles T, Muzykantov Vladimir R
Abstract excerpt
Thrombomodulin (TM) is a glycoprotein normally present in the membrane of endothelial cells that binds thrombin and changes its substrate specificity to produce activated protein C (APC) that has antithrombotic and anti-inflammatory features. To compensate for loss of endogenous TM in pathology, we have fused recombinant TM with single chain variable fragment (scFv) of an antibody to mouse platelet endothelial...
Topics
- Animals
- Blood Coagulation
- Cell Line
- Endothelial Cells
- Male
- Mice
- Mice, Inbred C57BL
- Mutation
- Oxidative Stress
- Partial Thromboplastin Time
- Platelet Endothelial Cell Adhesion Molecule-1
- Protein Binding