Article
Engineering a disulfide bond to stabilize the calcium-binding loop of activated protein C eliminates its anticoagulant but not its protective signaling properties.
The Journal of biological chemistry - 23 Mar 2007
Bae Jong-Sup, Yang Likui, Manithody Chandrashekhara, Rezaie Alireza R
Abstract excerpt
In addition to an anticoagulant activity, activated protein C (APC) also exhibits anti-inflammatory and cytoprotective properties. These properties may contribute to the beneficial effect of APC in treating severe sepsis patients. A higher incidence of bleeding because of its anticoagulant function has been found to be a major drawback of APC as an effective anti-inflammatory drug. In this study, we have prepared...
Topics
- Anti-Inflammatory Agents
- Anticoagulants
- Apoptosis
- Calcium
- Cell Line, Transformed
- Cell Movement
- Disulfides
- Humans
- Mutation
- Protein Binding
- Protein C