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Article

Eprobe mediated real-time PCR monitoring and melting curve analysis.

PloS one - 1 Jan 2013

Hanami Takeshi, Delobel Diane, Kanamori Hajime, Tanaka Yuki, Kimura Yasumasa, Nakasone Ayako, Soma Takahiro, Hayashizaki Yoshihide, Usui Kengo, Harbers Matthias

Abstract excerpt

Real-time monitoring of PCR is one of the most important methods for DNA and RNA detection widely used in research and medical diagnostics. Here we describe a new approach for combined real-time PCR monitoring and melting curve analysis using a 3' end-blocked Exciton-Controlled Hybridization-sensitive fluorescent Oligonucleotide (ECHO) called Eprobe. Eprobes contain two dye moieties attached to the same...

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Identifiers

PMID: 23951046
DOI: 10.1371/journal.pone.0070942
PMCID: PMC3737233

Genes

EGFR
KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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