Article
Energy transfer cassettes for facile labeling of sequencing and PCR primers.
Analytical biochemistry - 15 May 2001
Berti L, Xie J, Medintz I L, Glazer A N, Mathies R A
Abstract excerpt
Fluorescence energy transfer (ET) primers and terminators are the reagents of choice for multiplex DNA sequencing and analysis. We present here the design, synthesis and evaluation of a four-color set of ET cassettes, fluorescent labeling reagents that can be quantitatively coupled to a thiol-activated target through a disulfide exchange reaction. The ET cassette consists of a sugar-phosphate spacer with a FAM...
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