Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Enzyme-Free Detection of Mutations in Cancer DNA Using Synthetic Oligonucleotide Probes and Fluorescence Microscopy.

PloS one - 1 Jan 2015

Miotke Laura, Maity Arindam, Ji Hanlee, Brewer Jonathan, Astakhova Kira

Abstract excerpt

BACKGROUND: Rapid reliable diagnostics of DNA mutations are highly desirable in research and clinical assays. Current development in this field goes simultaneously in two directions: 1) high-throughput methods, and 2) portable assays. Non-enzymatic approaches are attractive for both types of methods since they would allow rapid and relatively inexpensive detection of nucleic acids. Modern fluorescence microscopy...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Line, Tumor
Share to Enzymes
Share to Fluorescent Dyes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Microscopy, Fluorescence

Share to Neoplasms

Share to Oligonucleotide Probes

Share to Oligonucleotides

Share to Proto-Oncogene Proteins B-raf

Source

Open in PubMed Open full text

Identifiers

PMID: 26312489
DOI: 10.1371/journal.pone.0136720
PMCID: PMC4552304

Genes

BRAF

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Enzyme-Free Detection of Mutations in Cancer DNA Using Synthetic Oligonucleotide Probes and Fluorescence Microscopy.PMID 26312489Read ↗

Programmable One-Pot Enzymatic Reaction for Direct Fluorescence Detection of Ultralow-Abundance Mutations in the DNA Duplex.Semantic proximity Read ↗

Solid-Phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence.Semantic proximity Read ↗

Blocking probe as a potential tool for detection of single nucleotide DNA mutations: design and performance.Semantic proximity Read ↗

An exciplex-based, target-assembled fluorescence system with inherently low background to probe for specific nucleic acid sequences.Semantic proximity Read ↗

Ultrasensitive detection of cancer-associated nucleic acids and mutations by primer exchange reaction-based signal amplification and flow cytometry.Semantic proximity Read ↗

Tweezer PCR: A Highly Specific Method for Accurate Identification of Low-Abundance Mutations.Semantic proximity Read ↗

Nanopore Identification of Single Nucleotide Mutations in Circulating Tumor DNA by Multiplexed Ligation.Semantic proximity Read ↗

DNA Strand Displacement Reaction: A Powerful Tool for Discriminating Single Nucleotide Variants.Semantic proximity Read ↗

Mutant enrichment with 3'-modified oligonucleotides a practical PCR method for detecting trace mutant DNAs.Semantic proximity Read ↗

Locked Nucleic Acid Technology for Highly Sensitive Detection of Somatic Mutations in Cancer.Semantic proximity Read ↗

Modified Unit-Mediated Strand Displacement Reactions for Direct Detection of Single Nucleotide Variants in Active Double-Stranded DNA.Semantic proximity Read ↗

Antiprimer quenching-based real-time PCR and its application to the analysis of clinical cancer samples.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.