Article
Detection of KRAS mutations using double-stranded toehold-exchange probes.
Biosensors & bioelectronics - 15 Jun 2016
Wu Zhenhua, Ma Tianle, Coll Jean-Luc, Liu Fangming, Zhang Honglian, Ma Yunfei, Wang Zhishuo, Jin Qinghui, Mao Hongju, Zhao Jianlong
Abstract excerpt
Detection of KRAS mutations in cancer tissues is immensely valuable for the identification of personalized genotype-based therapy. Here, we employed a double-stranded toehold-exchange probe, which is labeled with fluorescent molecules (FAM) and quenchers (Dabcyl), to detect KRAS mutations in cancer tissues. This probe was able to differentiate the intended mutation in a sample containing as little as 5% mutant...
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