Article
Genotyping by induced Förster Resonance Energy Transfer(iFRET) mechanism and simultaneous mutation scanning.
Human mutation - 1 Apr 2013
Masojć Bartłomiej, Górski Bohdan, van de Wetering Thierry, Dębniak Tadeusz, Cybulski Cezary, Jakubowska Anna, Mędrek Krzysztof, Rudnicka Helena, Dwight Zachary Lawrence, Lubiński Jan
Abstract excerpt
Multiple genotyping techniques were developed on the basis of real-time PCR. In this article, we present a genotyping technique extending the induced Förster resonance energy transfer (iFRET) mechanism in conjunction with simultaneous mutation scanning. Rapid, asymmetric PCR was performed with SYTO9, polymerase lacking 5 → 3 exonuclease activity, two primers, and a probe labeled with 6-Carboxy-X-rhodamine. Six...
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