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Article

Application of a novel strategy of engineering conditional alleles to a single exon gene, Sox2.

PloS one - 1 Jan 2012

Mandalos Nikolaos, Saridaki Marannia, Harper Jessica Lea, Kotsoni Anastasia, Yang Peter, Economides Aris N, Remboutsika Eumorphia

Abstract excerpt

BACKGROUND: The Conditional by Inversion (COIN) method for engineering conditional alleles relies on an invertible optimized gene trap-like element, the COIN module, for imparting conditionality. The COIN module contains an optimized 3' splice site-polyadenylation signal pair, but is inserted antisense to the target gene and therefore does not alter transcription, until it is inverted by Cre recombinase. In order...

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Identifiers

PMID: 23029233
DOI: 10.1371/journal.pone.0045768
PMCID: PMC3459942

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