Article
Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins.
Biotechnology letters - 1 Nov 2013
Larsen Sasha, Weaver Jun, de Sa Campos Katherine, Bulahan Rhobe, Nguyen Jackson, Grove Heather, Huang Amy, Low Lauren, Tran Namphuong, Gomez Seth, Yau Jennifer, Ilustrisimo Thomas, Kawilarang Jessica, Lau Jonathan, Tranphung Maivi, Chen Irene, Tran Christina, Fox Marcia, Lin-Cereghino Joan, Lin-Cereghino Geoff P
Abstract excerpt
Although Pichia pastoris is a popular protein expression system, it exhibits limitations in its ability to secrete heterologous proteins. Therefore, a REMI (restriction enzyme mediated insertion) strategy was utilized to select mutant beta-g alactosidase s upersecretion (bgs) strains that secreted increased levels of a β-galactosidase reporter. Many of the twelve BGS genes may have functions in intracellular...
Topics
- Genes, Reporter
- Metabolic Engineering
- Mutagenesis
- Mutation
- Pichia
- Recombinant Proteins
- beta-Galactosidase