Article
High-activity recombinant human carboxypeptidase B expression in Pichia pastoris through rational protein engineering and enhancing secretion from the Golgi apparatus to the plasma membrane.
Biotechnology journal - 1 May 2024
Li Jia, Shao Qinan, Xiang Yulong, Li Jianghua, Chen Jian, Du Guocheng, Kang Zhen, Wang Yang
Abstract excerpt
Human carboxypeptidase B1 (hCPB1) is vital for recombinant insulin production, holding substantial value in the pharmaceutical industry. Current challenges include limited hCPB1 enzyme activity. In this study, recombinant hCPB1 efficient expression in Pichia pastoris was achieved. To enhance hCPB...
Topics
- Humans
- Recombinant Proteins
- Protein Engineering
- Carboxypeptidase B
- Cell Membrane
- Golgi Apparatus
- Saccharomycetales
- Mutation
- Pichia
- Protein Sorting Signals
- Protein Transport