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Article

An episomal expression vector for screening mutant gene libraries in Pichia pastoris.

Plasmid - 1 Jul 2005

Lee Charles C, Williams Tina G, Wong Dominic W S, Robertson George H

Abstract excerpt

Screening mutant gene libraries for isolating improved enzyme variants is a powerful technique that benefits from effective and reliable biological expression systems. Pichia pastoris is a very useful organism to express proteins that are inactive in other hosts such as Escherichia coli and Saccharomyces cerevisiae. However, most P. pastoris expression plasmids are designed to integrate into the host chromosome...

Topics

Bleomycin
DNA Replication
Escherichia coli
Gene Expression Regulation, Fungal
Gene Library
Genetic Vectors
Mutation
Pichia
Plasmids
Promoter Regions, Genetic
Recombinant Proteins

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Identifiers

PMID: 15907541
DOI: 10.1016/j.plasmid.2004.12.001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-15907541-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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