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Article

Molecular genetic manipulation of Pichia pastoris SEC4 governs cell growth and glucoamylase secretion.

Biochemical and biophysical research communications - 4 Nov 2005

Liu Shi-Hwei, Chou Wei-I, Lin Shu-Chuan, Sheu Chia-Chin, Chang Margaret Dah-Tsyr

Abstract excerpt

We have previously engineered a recombinant Pichia pastoris GS115 transformant, MSPGA-7, harboring seven copies of glucoamylase (GA) fused with modified signal peptide. High yield secretion of GA was achieved as an extra copy of SEC4 was integrated to the transformant. To elucidate the physiological role of SEC4, a dominant-negative mutant of SEC4, SEC4(S28N), was overexpressed under the control of alchohol...

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Source

Identifiers

PMID: 16176807
DOI: 10.1016/j.bbrc.2005.08.234

Genes

AOX1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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