Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Molecular genetic manipulation of Pichia pastoris SEC4 governs cell growth and glucoamylase secretion.

Biochemical and biophysical research communications - 4 Nov 2005

Liu Shi-Hwei, Chou Wei-I, Lin Shu-Chuan, Sheu Chia-Chin, Chang Margaret Dah-Tsyr

Abstract excerpt

We have previously engineered a recombinant Pichia pastoris GS115 transformant, MSPGA-7, harboring seven copies of glucoamylase (GA) fused with modified signal peptide. High yield secretion of GA was achieved as an extra copy of SEC4 was integrated to the transformant. To elucidate the physiological role of SEC4, a dominant-negative mutant of SEC4, SEC4(S28N), was overexpressed under the control of alchohol...

Topics

Alcohol Oxidoreductases
Cell Proliferation
Cloning, Molecular
Fungal Proteins
Gene Dosage
Glucan 1,4-alpha-Glucosidase
Mutation
Pichia
Promoter Regions, Genetic
Saccharomyces cerevisiae Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

rab GTP-Binding Proteins

Source

Identifiers

PMID: 16176807
DOI: 10.1016/j.bbrc.2005.08.234

Genes

AOX1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Molecular genetic manipulation of Pichia pastoris SEC4 governs cell growth and glucoamylase secretion.PMID 16176807Read ↗

The membrane dynamics of pexophagy are influenced by Sar1p in Pichia pastoris.Semantic proximity Read ↗

High-level production of glucose oxidase in Pichia pastoris: Effects of Hac1p overexpression on cell physiology and enzyme expression.Semantic proximity Read ↗

Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris.Semantic proximity Read ↗

Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris.Semantic proximity Read ↗

High-activity recombinant human carboxypeptidase B expression in Pichia pastoris through rational protein engineering and enhancing secretion from the Golgi apparatus to the plasma membrane.Semantic proximity Read ↗

Overexpression of a dominant-negative allele of SEC4 inhibits growth and protein secretion in Candida albicans.Semantic proximity Read ↗

Anniversary of the discovery of sec mutants by Novick and Schekman.Semantic proximity Read ↗

The effect of the bgs13 mutation on the structure of the reporter protein beta-lactoglobulin: Influence on folding and aggregation in Pichia pastoris.Semantic proximity Read ↗

Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins.Semantic proximity Read ↗

Hydrolysis of GTP by Sec4 protein plays an important role in vesicular transport and is stimulated by a GTPase-activating protein in Saccharomyces cerevisiae.Semantic proximity Read ↗

Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene.Semantic proximity Read ↗

An episomal expression vector for screening mutant gene libraries in Pichia pastoris.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.