Article
Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae.
Journal of cell science - 1 Mar 1992
Chow T Y, Ash J J, Dignard D, Thomas D Y
Abstract excerpt
Utilizing a screening method designed for the identification of genes involved with enhanced protein secretion in Saccharomyces cerevisiae we identified a gene, which we named PSE-1 (Protein Secretion Enhancer). Overexpression of PSE-1 in a multi-copy plasmid, as shown by Northern hybridization,...
Topics
- Acid Phosphatase
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- DNA, Fungal
- Electrophoresis, Polyacrylamide Gel
- Fungal Proteins
- Genes, Fungal
- Mating Factor
- Membrane Transport Proteins
- Molecular Sequence Data
- Peptides
- Phenotype
- Plasmids
- Receptors, Cytoplasmic and Nuclear
- Restriction Mapping
- Saccharomyces cerevisiae