Article
The effect of the bgs13 mutation on the structure of the reporter protein beta-lactoglobulin: Influence on folding and aggregation in Pichia pastoris.
Protein expression and purification - 1 Dec 2023
Irshad Bushra, Lu Cai, Nederstigt Anneroos E, Hu Priscilla, Xue Liang, Lin-Cereghino Joan, Franz Andreas H, Harrison Joseph S, Lin-Cereghino Geoff P
Abstract excerpt
Pichia pastoris, a methylotrophic yeast used for recombinant protein expression, has the capability of performing many eukaryotic post-translational modifications, growing to high cell densities, and producing proteins in a cost-effective manner. However, P. pastoris's secretion properties are not always efficient, and its secretory pathway mechanisms have not been thoroughly elucidated. A previously identified...
Topics
- Saccharomycetales
- Biological Transport
- Lactoglobulins
- Mutation