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Article

Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis.

Applied and environmental microbiology - 1 Nov 2005

Colussi Paul A, Taron Christopher H

Abstract excerpt

The strong LAC4 promoter (P(LAC4)) from Kluyveromyces lactis has been extensively used to drive expression of heterologous proteins in this industrially important yeast. A drawback of this expression method is the serendipitous ability of P(LAC4) to promote gene expression in Escherichia coli. This can interfere with the process of assembling expression constructs in E. coli cells prior to their introduction into...

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Identifiers

PMID: 16269745
DOI: 10.1128/AEM.71.11.7092-7098.2005
PMCID: PMC1287696

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