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Article

A new type of gene-disruption cassette with a rescue gene for Pichia pastoris.

Biotechnology progress - 1 Sept 2017

Shibui Tatsuro, Hara Hiroyoshi

Abstract excerpt

Pichia pastoris has been used for the production of many recombinant proteins, and many useful mutant strains have been created. However, the efficiency of mutant isolation by gene-targeting is usually low and the procedure is difficult for those inexperienced in yeast genetics. In order to overcome these issues, we developed a new gene-disruption system with a rescue gene using an inducible Cre/mutant-loxP...

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PMID: 28840657
DOI: 10.1002/btpr.2541

Genes

AOX1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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