A new type of gene-disruption cassette with a rescue gene for Pichia pastoris.

doi:10.1002/btpr.2541

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Article

A new type of gene-disruption cassette with a rescue gene for Pichia pastoris.

Biotechnology progress - 1 Sept 2017

Shibui Tatsuro, Hara Hiroyoshi

Abstract excerpt

Pichia pastoris has been used for the production of many recombinant proteins, and many useful mutant strains have been created. However, the efficiency of mutant isolation by gene-targeting is usually low and the procedure is difficult for those inexperienced in yeast genetics. In order to overc...

Topics

Cloning, Molecular
Fungal Proteins
Gene Knockout Techniques
Integrases
Mutation
Pichia
Promoter Regions, Genetic
Recombinant Proteins

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