Article
Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris.
FEMS microbiology letters - 1 Nov 2006
Marx Hans, Sauer Michael, Resina David, Vai Marina, Porro Danilo, Valero Francisco, Ferrer Pau, Mattanovich Diethard
Abstract excerpt
The aim of the study was the identification, cloning and disruption of the GAS1 homologue of Pichia pastoris. Gas1p is a glycoprotein anchored to the outer layer of the plasma membrane through a glycosylphosphatidylinositol (GPI) anchor. Gas1p is a beta-1,3-glucanosyltransglycosylase (EC 2.4.1.-). This cross-linking enzyme highly affects the structure and permeability of the yeast cell wall. The gene coding for...
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