Article
Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.
PloS one - 1 Jan 2013
Zhang Jun-Hui, Jiang Yu-Yan, Lin Ying, Sun Yu-Fei, Zheng Sui-Ping, Han Shuang-Yan
Abstract excerpt
To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, As...
Topics
- Amino Acid Substitution
- Amino Acids
- Catalysis
- Enzyme Activation
- Fatty Acids
- Hydrophobic and Hydrophilic Interactions
- Lipase
- Lipids
- Models, Molecular
- Molecular Docking Simulation
- Mutation
- Protein Conformation
- Protein Engineering
- Rhizomucor
- Substrate Specificity