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Article

Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.

PloS one - 1 Jan 2013

Zhang Jun-Hui, Jiang Yu-Yan, Lin Ying, Sun Yu-Fei, Zheng Sui-Ping, Han Shuang-Yan

Abstract excerpt

To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257Leu were obtained...

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Identifiers

PMID: 23844120
DOI: 10.1371/journal.pone.0067892
PMCID: PMC3700896

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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