Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Engineering of a thermo-alkali-stable lipase from Rhizopus chinensis by rational design of a buried disulfide bond and combinatorial mutagenesis.

Journal of industrial microbiology & biotechnology - 1 Dec 2020

Wang Rui, Wang Shang, Xu Yan, Yu Xiaowei

Abstract excerpt

To improve the thermostability of the lipase (r27RCL) from Rhizopus chinensis through rational design, a newly introduced buried disulfide bond F223C/G247C was proved to be beneficial to thermostability. Interestingly, F223C/G247C was also found to improve the alkali tolerance of the lipase. Subsequently, six other thermostabilizing mutations from our previous work were integrated into the mutant F223C/G247C,...

Topics

Alkalies
Disulfides
Enzyme Stability
Lipase
Mutagenesis
Mutation
Protein Domains
Rhizopus
Temperature

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 33070231
DOI: 10.1007/s10295-020-02324-1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-33070231-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Engineering of a thermo-alkali-stable lipase from Rhizopus chinensis by rational design of a buried disulfide bond and combinatorial mutagenesis.PMID 33070231Read ↗

[Improving the thermal stability of Bacillus subtilis lipase based on multiple computational design strategies].Semantic proximity Read ↗

Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide.Semantic proximity Read ↗

Discovery of the Key Mutation Site Influencing the Thermostability of Thermomyces lanuginosus Lipase by Rosetta Design Programs.Semantic proximity Read ↗

Hook loop dynamics engineering transcended the barrier of activity-stability trade-off and boosted the thermostability of enzymes.Semantic proximity Read ↗

The AXXXA motif: A rational design strategy and experimental studies to enhance both activity and thermostability of BTL2 lipase.Semantic proximity Read ↗

[Enhanced thermostability of Rhizopus chinensis lipase by error-prone PCR].Semantic proximity Read ↗

Computational approach for designing thermostable Candida antarctica lipase B by molecular dynamics simulation.Semantic proximity Read ↗

Structure-based engineering of methionine residues in the catalytic cores of alkaline amylase from Alkalimonas amylolytica for improved oxidative stability.Semantic proximity Read ↗

Enhancing the thermostability of a cold-active lipase from Penicillium cyclopium by in silico design of a disulfide bridge.Semantic proximity Read ↗

Y12F mutation in Pseudomonas plecoglossicida S7 lipase enhances its thermal and pH stability for industrial applications: a combination of in silico and in vitro study.Semantic proximity Read ↗

Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design.Semantic proximity Read ↗

Significant improvement of the thermostability and catalytic efficiency of β-glucosidase Bgl59 via cysteine engineering and rational design.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.