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Article

High-throughput screening of B factor saturation mutated Rhizomucor miehei lipase thermostability based on synthetic reaction.

Enzyme and microbial technology - 10 May 2012

Zhang Jun-hui, Lin Ying, Sun Yu-fei, Ye Yan-rui, Zheng Sui-ping, Han Shuang-yan

Abstract excerpt

Conventional lipase screening methods are mostly based on hydrolytic activity, which may not always be the best method to assess the enzyme activity, especially for evaluating synthetic activity. Here we developed a high throughput and visual method to screen clones with high synthetic activity a...

Topics

Biotechnology
Caprylates
Culture Media
Enzyme Stability
Enzymes, Immobilized
Esterification
Ethanol
High-Throughput Screening Assays
Hot Temperature
Hydrogen-Ion Concentration
Lipase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Rhizomucor

Source

Identifiers

PMID: 22500900
DOI: 10.1016/j.enzmictec.2012.03.002

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-22500900-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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