Article
Targeted mutations and MD simulations of a methanol-stable lipase YLIP9 from Yarrowia lipolytica MSR80 to develop a biodiesel enzyme.
International journal of biological macromolecules - 1 Nov 2017
Syal Poonam, Verma Ved Vrat, Gupta Rani
Abstract excerpt
Biodiesel, an environment friendly alternative for fuels, contains methyl esters of long-chain fatty acids. Our group has reported a methanol-stable YLIP9 from Yarrowia lipolytica MSR80 that shows poor catalysis of long-chain fatty acids. To shift its substrate specificity, residues within lid and binding pocket were identified for sequential mutations using YLIP2 as the template. Of the two point mutations...
Topics
- Amino Acid Sequence
- Biofuels
- Enzyme Activation
- Enzyme Stability
- Lipase
- Methanol
- Molecular Dynamics Simulation
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Substrate Specificity