Article
New engineered Geobacillus lipase GD-95RM for industry focusing on the cleaner production of fatty esters and household washing product formulations.
World journal of microbiology & biotechnology - 27 Feb 2020
Druteika Gytis, Sadauskas Mikas, Malunavicius Vilius, Lastauskiene Egle, Statkeviciute Roberta, Savickaite Agne, Gudiukaite Renata
Abstract excerpt
This study presents a new microbial lipolytic enzyme GD-95RM designed via random mutagenesis using previously characterized GD-95 lipase as a template. The improvement in activity of GD-95 lipase was caused by E100K, F154V and V174I mutations. Compared with GD-95 lipase, the GD-95RM lipase had 1.3-fold increased specific activity (2000 U/mg), demonstrated resistance to higher temperatures (75-85 °C), had fourfold...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.