Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide.

doi:10.1016/j.enzmictec.2021.109870

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Article

Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide.

Enzyme and microbial technology - 1 Oct 2021

Tian Miao, Huang Shaowei, Wang Zhiyuan, Fu Junying, Lv Pengmei, Miao Changlin, Liu Tao, Yang Lingmei, Luo Wen

Abstract excerpt

The propeptide is a short sequence that facilitates protein folding. In this study, four highly active Rhizomucor miehei lipase (RML) mutants were obtained through saturation mutagenesis at three propeptide positions: Ser8, Pro35, and Pro47. The enzyme activities of mutants P35 N, P47 G, P47 N, a...

Topics

Lipase
Mutation
Protein Folding
Rhizomucor

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