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Article

Directed co-evolution of an endoglucanase and a β-glucosidase in Escherichia coli by a novel high-throughput screening method.

Chemical communications (Cambridge, England) - 21 Aug 2013

Liu Min, Gu Jiali, Xie Wenping, Yu Hongwei

Abstract excerpt

A novel high-throughput screening method for directed co-evolution of endoglucanase and β-glucosidase was constructed based on the synergy of the two enzymes using a bicistronic operon. To our knowledge, this is the first report of directed co-evolution of two cellulosic enzymes using an insoluble screening substrate via their synergistic reactions.

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PMID: 23841109
DOI: 10.1039/c3cc42485e

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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