Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells.

Journal of molecular biology - 27 Mar 2015

Boock Jason T, King Brian C, Taw May N, Conrado Robert J, Siu Ka-Hei, Stark Jessica C, Walker Larry P, Gibson Donna M, DeLisa Matthew P

Abstract excerpt

Heterologous expression of many proteins in bacteria, yeasts, and plants is often limited by low titers of functional protein. To address this problem, we have created a two-tiered directed evolution strategy in Escherichia coli that enables optimization of protein production while maintaining high biological activity. The first tier involves a genetic selection for intracellular protein stability that is based...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arginine
Share to Cellulase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fusarium

Share to Protein Engineering

Share to Protein Folding

Share to Protein Stability

Share to Quality Control

Share to Solubility

Source

Open in PubMed Open full text

Identifiers

PMID: 25591491
DOI: 10.1016/j.jmb.2015.01.003
PMCID: PMC4576832

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells.PMID 25591491Read ↗

Protein engineering.Semantic proximity Read ↗

Global analysis of protein folding using massively parallel design, synthesis, and testing.Semantic proximity Read ↗

High-throughput Activity Reprogramming of Proteases (HARP)Semantic proximity Read ↗

AI-directed gene fusing prolongs the evolutionary half-life of synthetic gene circuitsSemantic proximity Read ↗

Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability.Semantic proximity Read ↗

Selection of chromosomal DNA libraries using a multiplex CRISPR system.Semantic proximity Read ↗

Improved bacterial recombineering by parallelized protein discovery.Semantic proximity Read ↗

Engineering Strategies to Overcome the Stability-Function Trade-Off in Proteins.Semantic proximity Read ↗

Deep mutational scanning of essential bacterial proteins can guide antibiotic development.Semantic proximity Read ↗

The F-box protein gene exo-1 is a target for reverse engineering enzyme hypersecretion in filamentous fungi.Semantic proximity Read ↗

Improved bacterial recombineering by parallelized protein discoverySemantic proximity Read ↗

Programming cells by multiplex genome engineering and accelerated evolution.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.