Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli.

ACS synthetic biology - 15 Jul 2016

Bassalo Marcelo C, Garst Andrew D, Halweg-Edwards Andrea L, Grau William C, Domaille Dylan W, Mutalik Vivek K, Arkin Adam P, Gill Ryan T

Abstract excerpt

Methods for importing heterologous genes into genetically tractable hosts are among the most desired tools of synthetic biology. Easy plug-and-play construction methods to rapidly test genes and pathways stably in the host genome would expedite synthetic biology and metabolic engineering applications. Here, we describe a CRISPR-based strategy that allows highly efficient, single step integration of large pathways...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Endonucleases

Share to Genetic Engineering

Share to Metabolic Engineering

Share to Metabolic Networks and Pathways

Share to MutS DNA Mismatch-Binding Protein

Share to RNA, Guide, CRISPR-Cas Systems

Source

Identifiers

PMID: 27072506
DOI: 10.1021/acssynbio.5b00187

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli.PMID 27072506Read ↗

Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods.Semantic proximity Read ↗

Techniques for Large-Scale Bacterial Genome Manipulation and Characterization of the Mutants with Respect to In Silico Metabolic Reconstructions.Semantic proximity Read ↗

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9.Semantic proximity Read ↗

A highly efficient Cre-based Clostridial workflow for genomic integration and expression of large biosynthetic pathwaysSemantic proximity Read ↗

MIDAS: A Modular DNA Assembly System for Synthetic Biology.Semantic proximity Read ↗

An efficient cre‐based workflow for genomic integration and expression of large biosynthetic pathways in Eubacterium limosumSemantic proximity Read ↗

Combinatorial pathway engineering using type I-E CRISPR interference.Semantic proximity Read ↗

Tools for genome-wide strain design and construction.Semantic proximity Read ↗

Selection of chromosomal DNA libraries using a multiplex CRISPR system.Semantic proximity Read ↗

Lessons from metabolic engineering for functional genomics and drug discovery.Semantic proximity Read ↗

Multiplex genome editing by natural transformation.Semantic proximity Read ↗

mpCRISTAR: Multiple Plasmid Approach for CRISPR/Cas9 and TAR-Mediated Multiplexed Refactoring of Natural Product Biosynthetic Gene Clusters.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.