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Article

Inducible directed evolution of complex phenotypes in bacteria.

Nucleic acids research - 10 Jun 2022

Al'Abri Ibrahim S, Haller Daniel J, Li Zidan, Crook Nathan

Abstract excerpt

Directed evolution is a powerful method for engineering biology in the absence of detailed sequence-function relationships. To enable directed evolution of complex phenotypes encoded by multigene pathways, we require large library sizes for DNA sequences >5-10 kb in length, elimination of genomic hitchhiker mutations, and decoupling of diversification and screening steps. To meet these challenges, we developed...

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Identifiers

PMID: 35150576
DOI: 10.1093/nar/gkac094
PMCID: PMC9177967

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