Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop.

Abstract excerpt

Protein engineers have widely adopted directed evolution as a design algorithm, but practitioners have not come to a consensus about the best method to evolve protein molecular recognition. We previously used DNA shuffling to direct the evolution of Escherichia coli beta-glucuronidase (GUS) variants with increased beta-galactosidase activity. Epistatic (synergistic) mutations in amino acids 557, 566, and 568,...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Algorithms
• Share to Glucuronidase
• Share to Kinetics