Article
Zooming in on single active genes in living mammalian cells.
Histochemistry and cell biology - 1 Jul 2013
Yunger Sharon, Kalo Alon, Kafri Pinhas, Sheinberger Jonathan, Lavi Erez, Neufeld Noa, Shav-Tal Yaron
Abstract excerpt
The kinetic aspects of RNA polymerase II as it transcribes mRNA have been revealed over the past decade by use of live-cell imaging and kinetic analyses. It is now possible to visualize polymerase molecules in action, and most importantly to detect and follow the mRNA product as it is generated in real time on active genes. Questions such as the speed at which mRNAs are transcribed or the number of polymerases...
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