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Article

Quantifying the transcriptional output of single alleles in single living mammalian cells.

Nature protocols - 1 Feb 2013

Yunger Sharon, Rosenfeld Liat, Garini Yuval, Shav-Tal Yaron

Abstract excerpt

Transcription kinetics of actively transcribing genes in vivo have generally been measured using tandem gene arrays. However, tandem arrays do not reflect the endogenous state of genome organization in which genes appear as single alleles. Here we present a robust technique for the quantification of mRNA synthesis from a single allele in real time in single living mammalian cells. The protocol describes how to...

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Share to Alleles
Share to Cloning, Molecular
Share to Gene Expression Regulation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to In Situ Hybridization, Fluorescence

Share to RNA, Messenger

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Share to Transcription, Genetic

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Identifiers

PMID: 23424748
DOI: 10.1038/nprot.2013.008
PMCID: PMC3597184

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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